Widely used tests for the detection of platelet antibodies in Korea include platelet suspension immunofluorescence test(PSIFT), enzyme immunoassay and mixed passive hemagglutination (MPHA). In these tests, removal of HLA antigens from platelet
are
required to detect platelet-specific antibodies. Modified antigen capture ELISA(MACE) is known to be very sensitive for the detection of platelet-specific antibodies, in which specific platelet glycoprotein, captured by the monoclonal ntibody is
used as
a target antigen. MACE is very useful for the detection of plateletspecific alloantibodies in neonatal alloimmune thrombocytopenia (NAIT) and posttransfusion purpura(PTP). We employed MACE in our laboratory, using AP2(anti-GPIIb/IIIa,
monoclonal),
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sera(anti-PlAl), 90-545 sera (antiHLA-B51+52) and LYS sera(multispecific HLA antibodies). LYS sera had been used as our positive control(1:120) in MPHA. Platelet from PlAl(+), HLA-B51, blood group O healthy male donor, gave positive result with
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sera
(1:40) and negative result with 90-545 sera in MACE. With LYS sera. MACE showed negative in 1:120, but positive in 1:20. So LYS sere was thought to contain strong multispecific HLA antibodies and relatively weak antibody(-ies) reacting with
GPIIb/IIIa.
Further studies employing different monoclonal antibodies, such as antiGPIb/Ⅸ, -GPIa/IIa and-GPIV are under way.
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